ABSTRACT
RESUMEN Objetivo Establecer la correlación entre los métodos de Wintrobe y de micro-hematocrito Métodos Se tomaron 407 pacientes asistentes a un laboratorio de tercer nivel a quienes en forma simultánea se les realizó las dos pruebas en estudio. Resultados Mediante el un método estadístico de regresión lineal se encontró un coeficiente de correlación de 0,99. Conclusión Ambos métodos pueden contribuir al análisis clínico de los pacientes a quienes se les solicitó la prueba con fines de diagnóstico, control y seguimiento de diferentes patologías.(AU)
ABSTRACT Objective To establish the correlation between the methods of Wintrobe and micro-hematocrit Methods 407 patients attendees were taken laboratory to third level simultaneo usly tests were performed in the study. Results Using a statistical method of linear regression correlation coefficient of 0,99 was found. Conclusion Both methods can contribute to the clinical analysis of patients who were asked to test for diagnosis or control of different pathologies.(AU)
Subject(s)
Humans , Blood Sedimentation , Hematocrit/methods , Hematologic Diseases/diagnosis , Linear Models , Epidemiology, DescriptiveABSTRACT
The blood is an important liquid connective tissue flow in body and performs the role of distribution of oxygen to various tissues, taken out carbon dioxide and maintains the health status of an organism. Any change in the blood components can cause adverse effects on the body. The effect of mercuric chloride has been evaluated on blood in albino rats (Rattus norvegicus). The albino rats were treated with mercuric chloride 0.926 mg kg-1 body wt. for acute (1 day) and 0.044 mg kg-1 body wt. for sub-acute (7, 14 and 21 day) sets after calculating LD50 (9.26 mg kg-1 body wt.). Major changes have been observed in the form of enhanced clotting time (CT) and bleeding time (BT) due to toxic effect of mercuric chloride on haemopoietic system along with decrease in the total erythrocyte count (TEC) and haemoglobin concentration (Hb. conc.). The changes in erythrocyte count and haemoglobin concentration have been correlated with cytotoxic effect of mercuric chloride on erythropoiesis. However, the intoxication of mercuric chloride on total leukocyte count (TLC) and erythrocyte sedimentation rate (ESR) has been observed to be significantly increased after acute and sub-acute treatments due to leucocytosis and rouleux formation. Moreover, the present observations highlight dose dependent toxicity.
ABSTRACT
The ESR is one of the oldest laboratory test still in use. Although it lacks specificity in diagnosis, it can be effective for monitoring disease activity and following-up. The Westergren method is used for reference method, however coefficient of variation has been described 0.8% to 22.9% according to the literature. Since the ESR was invented in 1921, measurement technique has developed and automated measurement is introduced. We analyzed one hundred forty-three patient samples using SEDIsystem' automated ESR measuring system and compared with modified Westergren and Wintrobe methods. Comparison between SEDIsystem' and modified Westergren for ESR measurement yields the following regression equation; y = 0.863x - 1.69 (r=0.830), SEDIsystem'M and Wintrobe y'= 1.14x - 14.7 (r=0.789), rcspectively. We repeated measurement to evaluate reliability, results are not significant in statistically. In conclusion, SEDIsysten' automated ESR measurement correlated with modified Westergren and Wintro" ; methods, reveal reliable results after 4 hours and can report rapidly for large samples. Thus, these results indicate that SEDIsystenT"' automated ESR measurement may be useful tool for clinical practice.